hdac5 20458s cst wb  (Cell Signaling Technology Inc)

Journal: Journal of Neuroinflammation

Article Title: Upregulated astrocytic HDAC7 induces depression-like disorders via deacetylating PINK1 and inhibiting mitophagy

doi: 10.1186/s12974-025-03603-3

Figure Lengend Snippet: Increased expression of HDAC7 in astrocytes correlates with autophagy impairments in LPS-induced mouse model of depression. A and B Western blotting analysis and quantification of HDAC4, HDAC5, HDAC7, and HDAC9 in mouse brain hippocampus tissue. N = 3 mice per group, unpaired Student’s t test. C - E Immunofluorescence analysis of HDAC7 colocalization and expression changes with: C astrocytes, D microglia, E neurons in the hippocampus of LPS- and saline-treated mice. N = 3 mice per group, unpaired Student’s t test. F - I Western blotting analysis and quantification of HDAC7 in primary cultured cells as indicated: F astrocytes, G microglia, H neurons. N = 3 per group, unpaired Student’s t test. J - M Immunofluorescence staining and correlation analysis of HDAC7 with autophagy markers LC3B ( J ) or LAMP1 ( L ) in GFAP positive cells in the hippocampus of LPS- and saline-treated mice. Correlation between HDAC7 and LC3B or LAMP1 are analyzed with simple linear regression. N = 30 cells from three mice. Data are presented as mean ± SEM, * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: HDAC5 , 20458S , CST , WB: 1:1000.

Techniques: Expressing, Western Blot, Immunofluorescence, Saline, Cell Culture, Staining